Advanced Light Microscopy Node Bilbao Spain

The Bilbao Advanced Light Microscopy Node (ALM@BIO) is a joint initiative of the imaging platforms of the Instituto Biofisika (CSIC, UPV/EHU) and Achucarro Basque Center for Neuroscience, both located in the Science Park of the University of the Basque Country (UPV/EHU) in the metropolitan area of Bilbao. The Node is focused on quantitative imaging of cellular events such as molecular interactions and biophysical studies such as lipid-mediated protein interactions or lipid-order imaging of model membranes and cells; and on the neurobiology of neuron-glia interactions.

Specialties and expertise of the Node

ALM@BIO provides assistance during the whole experimentation process: experiment design, training, image analysis and storage. The Node offers experimental configurations that are not available commercially and home-made application-tailored software with a particular emphasis on membrane-related measurements and neurobioimaging, such as:

  • Spatio-temporal study of intracellular molecular interactions (FRET-FLIM)
  • Ligand-receptor binding (FCS)
  • Lipid-mediated protein interactions (scanning FCS)
  • Intact-cell imaging of lipid concentration at cellular membranes
  • Super resolution imaging of primary cells and slices of the CNS
  • Live imaging of genetically encoded calcium indicatora

Offered Technologies:

  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence cross-correlation spectroscopy (FCCS)
  • Fluorescence-lifetime imaging microscopy (FLIM)
  • Fluorescence resonance energy transfer (FRET)
  • Stimulated emission depletion microscopy (STED)

Instrument highlights

Special features of the instruments at the Bilbao Node are:

  • Tailor-made set-up for scanning FCCS for the study of lipid-mediated protein interactions
  • Live cell imaging capabilities: CO2 chamber and resonant scanner
  • In-house developed analysis software tailored to each application
  • Stimulated emission depletion microscopy (Gated CW-STED)

Contact details

Artur Escalada
CSIC - The Spanish Scientific Research Council
+34 946015698
Instituto Biofisika (CSIC, UPV/EHU)

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