Wageningen Imaging and Spectroscopy Hub (WISH) - ALM and Molecular Imaging Node Wageningen

The WISH – ALM and Molecular Imaging Node in Wageningen joins the outstanding scientific and technical excellence of three participating expertise groups specialized in light microscopy, microspectroscopy, and magnetic resonance. This Node applies advanced imaging technologies to plant research within a multidisciplinary research environment of cell & developmental biologists, (bio-) physicists and biochemists. Advanced instrumentation has been developed for the particular purpose of studying photosynthesis in vivo with the use of ultrafast fluorescence at the level of individual cells and chloroplasts.

Specialties and expertise of the Node

Spectroscopic investigations of energy transfer within photosynthetic complexes; functional imaging of membrane associated protein complexes involved in plant signaling and photosynthesis; and the visualization of plant developmental processes like cell division.

Offered Technologies:

Technologies Euro-BioImaging
Laser scanning confocal microscopy (LSCM/CLSM)
Spinning disk confocal microscopy (SDCM)
Total internal reflection fluorescence microscopy (TIRF)
Two-photon microscopy (2P)
Single Molecula localisation microscopy (SMLM)
Fluorescence Resonance Energy Transfer (FRET)
Fluorescence Recovery After Photobleaching (FRAP)
Fluorescence (cross)-correlation spectroscopy (FCS/FCCS)
Fluorescence Lifetime Imaging (FLIM)

Contact details

Johannes Hohlbein
Associate Professor, Laboratory of Biophysics
Wageningen University & Reserach

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